Addgene - Agrp-pGL3 Plasmid Data

Plasmid 17552: Agrp-pGL3

Gene/insert name:
Agrp
Insert size:
396
Species:
M. musculus (mouse)
Entrez Gene:
Agrp (Art, Agrt)
Mutation:
Contains ~400bp of the mouse Pomc gene promoter, including the STAT3/Foxo binding site
Vector backbone:
pGL3
(Search Vector Database)
Vector type:
Luciferase
Backbone size w/o insert (bp):
4818
Cloning site 5':
?
Site destroyed during cloning:
Unknown
Cloning site 3':
?
Site destroyed during cloning:
Unknown
5' sequencing primer:
? List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
Unknown
Sequence: View sequences (2)
Principal Investigator:
Domenico Accili
Terms and Licenses:
MTA
Luciferase Limited Use Label License

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Forkhead protein FoxO1 mediates Agrp-dependent effects of leptin on food intake. Kitamura et al (Nat Med. 2006 May . 12(5):534-40. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 17552" in your Materials and Methods section.