|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||17552||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4818
Growth in Bacteria
SpeciesM. musculus (mouse)
Insert Size (bp)396
MutationContains ~400bp of the mouse Pomc gene promoter, including the STAT3/Foxo binding site
Entrez GeneAgrp (a.k.a. Ag, Agrt, Art)
- Cloning method Restriction Enzyme
- 5′ cloning site ? (unknown if destroyed)
- 3′ cloning site ? (unknown if destroyed)
- 5′ sequencing primer ? (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:Agrp-pGL3 was a gift from Domenico Accili (Addgene plasmid # 17552 ; http://n2t.net/addgene:17552 ; RRID:Addgene_17552)
For your References section:Forkhead protein FoxO1 mediates Agrp-dependent effects of leptin on food intake. Kitamura T, Feng Y, Kitamura YI, Chua SC, Xu AW, Barsh GS, Rossetti L, Accili D. Nat Med. 2006 May . 12(5):534-40. 10.1038/nm1392 PubMed 16604086