|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||17553||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4818
Vector typeMammalian Expression, Luciferase
Growth in Bacteria
Gene/Insert namePomc promoter
Alt name-646 to +65
SpeciesM. musculus (mouse)
Insert Size (bp)708
Entrez GenePomc (a.k.a. ACTH, BE, Beta-LPH, Clip, Gamma-LPH, Npp, Pomc-1, Pomc1, alpha-MSH, alphaMSH, beta-MSH, gamma-MSH)
- Promoter Pomc
- Cloning method Restriction Enzyme
- 5′ cloning site ? (unknown if destroyed)
- 3′ cloning site ? (unknown if destroyed)
- 5′ sequencing primer RVprimer3
- 3′ sequencing primer LucNRev (Common Sequencing Primers)
Addgene's sequence shows a C->T mutation in nt 129 of POMC. The depositing scientist is aware of this mutation and has found that it does not alter the promoter activity.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:Pomc-pGL3 was a gift from Domenico Accili (Addgene plasmid # 17553 ; http://n2t.net/addgene:17553 ; RRID:Addgene_17553)
For your References section:Forkhead protein FoxO1 mediates Agrp-dependent effects of leptin on food intake. Kitamura T, Feng Y, Kitamura YI, Chua SC, Xu AW, Barsh GS, Rossetti L, Accili D. Nat Med. 2006 May . 12(5):534-40. 10.1038/nm1392 PubMed 16604086