TLX2-lux
(Plasmid
#17567)
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Depositing Lab
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Depositing OrganizationSinai Health System
Located in Canada -
Publication
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Citations
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 17567 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 * | |
| DNA | 17567-D50 | 50 μg of DNA in Tris buffer | $495 * | ||
| DNA | 17567-D100 | 100 μg of DNA in Tris buffer | $585 * | ||
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Backbone
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Vector backbonepGL2-Basic
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Backbone manufacturerPromega
- Backbone size w/o insert (bp) 5600
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Vector typeMammalian Expression, Luciferase
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTLX2 promoter
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Alt nameTLX2-pGL2
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Alt nameTLX2
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SpeciesM. musculus (mouse)
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Insert Size (bp)1700
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Entrez GeneTlx2 (a.k.a. Enx, NCX, Ncx1, Tlx1l1, Tlx1l2, Hox11l1, Hox11L.1)
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Tag
/ Fusion Protein
- Luciferase (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NcoI (destroyed during cloning)
- 3′ cloning site BamHI (destroyed during cloning)
- 5′ sequencing primer na
- 3′ sequencing primer LucNrev
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
See author's map.
DNA (Catalog # 17567-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 17567-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
TLX2-lux was a gift from Jeff Wrana (Addgene plasmid # 17567 ; http://n2t.net/addgene:17567 ; RRID:Addgene_17567) -
For your References section:
The Tlx-2 homeobox gene is a downstream target of BMP signalling and is required for mouse mesoderm development. Tang SJ, Hoodless PA, Lu Z, Breitman ML, McInnes RR, Wrana JL, Buchwald M. Development. 1998 May . 125(10):1877-87. PubMed 9550720
Map uploaded by the depositor.
