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iDuet101a
(Plasmid #17629)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 17629 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    Duet011
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Hygromycin ; GFP

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TetO7 - EF-1a promoter - GFP - PGK promoter - Hygro

Cloning Information

Resource Information

Depositor Comments

The full plasmid sequence is digitally assembled and may contain slight discrepancies, please check the Addgene QC sequencing.

Lentiviral dual promoter/dual-gene expression vector is dubbed Duet (for Dual-gene, excisable transgene).

Tet-Inducible Vector. An EF1{alpha}-containing inducible vector, iDuet101A, was constructed by replacing the UBC promoter in iDuet011A with the EF1{alpha} promoter .

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    iDuet101a was a gift from Linzhao Cheng (Addgene plasmid # 17629 ; http://n2t.net/addgene:17629 ; RRID:Addgene_17629)
  • For your References section:

    Inducible and reversible transgene expression in human stem cells after efficient and stable gene transfer. Zhou BY, Ye Z, Chen G, Gao ZP, Zhang YA, Cheng L. Stem Cells. 2007 Mar . 25(3):779-89. 10.1634/stemcells.2006-0128 PubMed 17158240