Addgene - pBABE-hygro Plasmid Data

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Related Plasmids

From this article

Bob Weinberg Lab Plasmids

This is commonly
requested with

pBABE-neo

pBABE-puro

pBABE-zeo ( pBABE-bleo)

pBABE GFP

pBABE-hygro- hTERT

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Plasmid 1765: pBABE-hygro

Gene/insert name:		none
Insert size (bp):		Unknown
Vector backbone:		pBABE-hygro (Search Vector Database)
Type of vector:		Mammalian expression,Retroviral
Backbone size (bp):		5558
5' Sequencing primer:		pBABE 5' (List of Sequencing Primers)
3' Sequencing primer:		pBABE 3'
Bacteria resistance:		Ampicillin
High or low copy:		High Copy
Grow in standard E. coli @ 37C:		Yes
Selectable markers:		Hygromycin
Sequence:		View sequence
Author's Map:		View map
Plasmid Provided In:		DH5a
Principal Investigator:		Bob Weinberg
Additional Documents:		pBABE protocol (PDF)
Terms and Licenses:		MTA

Comments: Morgenstern JP, Land H., 1990, Nucleic Acids Research 18(12):3587-96.

If you are using the pBABE protocol from the Weinberg Lab to generate virus, please note that Addgene supplies pCL-Eco (#12371), VSV-G (#8454), and a gag/pol expression vector (#8455).

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Click on map to enlarge

Selected features

5_LTR2

12 - 479

3MoMuLV_LTR

18 - 479

psi_plus_pack

549 - 1350

pBABE_5_primer

1318 - 1334

pBABE_3_primer

1428 - 1408

SV40_enhancer

1629 - 1414

SV40_promoter

1426 - 1694

SV40_origin

1593 - 1670

SV40_promoter

1546 - 1748

SV40pro_F_primer

1655 - 1674

Orf frame 3

1752 - 2801

hygroB

1785 - 2792

3MoMuLV_LTR

2839 - 3432

5_LTR2

2881 - 3432

pBR322_origin

4408 - 3789

Ampicillin

5430 - 4570

AmpR_promoter

5500 - 5472

Unique restriction sites

SpeI		616
BamHI		1355
EcoRI		1379
SalI		1397
StuI		1726
HindIII		1743
NdeI		2220
SacII		2550
ClaI		2795
NheI		2869
NotI		4522
FspI		4865

Please acknowledge the principal investigator if you use this plasmid in a publication.

Also, please include the text "Addgene plasmid 1765" in your Materials and Methods section. This information allows Addgene to create a link from the plasmid page to your publication.