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Plasmid 1765: pBABE-hygro
  • Retroviral vector for cloning and expressing your gene of interest. Hygromycin selection.

  • None

  • pBABE-hygro
    (Search Vector Database)

  • Mammalian Expression, Retroviral

  • 5558

  • pBABE 5' List of Sequencing Primers

  • pBABE 3'

  • Ampicillin

  • DH5alpha

  • 37

  • High Copy

  • Hygromycin

  • View sequences (5)
  • View map

  • pBABE protocol (application/pdf)

  • Notes from Addgene (2)

  • Hartmut Land

  • Jay Morgenstern

  • Bob Weinberg

  • UBMTA

Comments: 

Please acknowledge Jay Morgenstern and Hartmut Land and cite the following article if you use this plasmid in a publication:
Morgenstern JP, Land H., 1990, Nucleic Acids Research 18(12):3587-96.

If you are using the pBABE protocol from the Weinberg Lab to generate virus, please note that the Weinberg Lab recommends using pUMVC (Addgene #8449) and VSV-G (#8454) for packaging. The pCL-Eco plasmid listed in their protocol should be substituted with pUMVC.

Please note that the sequence from which the Addgene map was generated is an estimate of the real sequence based on how the vector was assembled. We encourage scientists to test enzymes before using them for cloning.

Addgene has sequenced a portion of this plasmid for verification. Full plasmid sequence is available only if provided by the depositing laboratory.

Article: Advanced mammalian gene transfer: high titre retroviral vectors with multiple drug selection markers and a complementary helper-free packaging cell line. Morgenstern et al (Nucleic Acids Res. 1990 Jun 25;18(12):3587-96. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 1765" in your Materials and Methods section.

Pending

This plasmid is currently not available for distribution from Addgene. Please check back later. If you have further questions, please contact Addgene at help@addgene.org.