Addgene - pBABE-hygro Plasmid Data

Plasmid 1765: pBABE-hygro

Gene/insert name:
None
Vector backbone:
pBABE-hygro
(Search Vector Database)
Vector type:
Mammalian Expression, Retroviral
Backbone size (bp):
5558
5' sequencing primer:
pBABE 5' List of Sequencing Primers
3' sequencing primer:
pBABE 3'
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Hygromycin
Sequence: View sequences (4)
Map:
View map
Supplemental document:
pBABE protocol (application/pdf)
Supplemental document:
Notes from Addgene (2)
Principal Investigator:
Bob Weinberg
Terms and Licenses:
MTA

Comments:

Morgenstern JP, Land H., 1990, Nucleic Acids Research 18(12):3587-96.

If you are using the pBABE protocol from the Weinberg Lab to generate virus, please note that the Weinberg Lab recommends using pUMVC (Addgene #8449) and VSV-G (#8454) for packaging. The pCL-Eco plasmid listed in their protocol should be substituted with pUMVC.

Please note that the sequence from which the Addgene map was generated is an estimate of the real sequence based on how the vector was assembled. We encourage scientists to test enzymes before using them for cloning.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 1765" in your Materials and Methods section.