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CBFRE-EGFP
(Plasmid #17705)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 17705 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    na
  • Backbone size w/o insert (bp) 3600
  • Vector type
    Mammalian Expression, Adenoviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CBFRE
  • Alt name
    CBF1-responsive element
  • Insert Size (bp)
    1150
  • Tag / Fusion Protein
    • EGFP (C terminal on insert)

Cloning Information

Resource Information

Depositor Comments

CBFRE: four CBF1-binding elements from EBV (see Hsieh MCB 1996, 16:952) and the basal simian virus 40 (SV40) promoter upstream of EGFP. The sequence of the CBF1 binding element is GATCTGGAGTAACACGCCGTGGGAAAAAATTTATG. The binding site within this sequence is GTGGGAA.

A SmaI+XbaI double digest should yield 3.6kb and approx. 1.2kb bands. See Reviews link in right-hand column to view Addgene's diagnostic digest of this plasmid.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CBFRE-EGFP was a gift from Nicholas Gaiano (Addgene plasmid # 17705 ; http://n2t.net/addgene:17705 ; RRID:Addgene_17705)
  • For your References section:

    Differential Notch signalling distinguishes neural stem cells from intermediate progenitors. Mizutani K, Yoon K, Dang L, Tokunaga A, Gaiano N. Nature. 2007 Sep 20. 449(7160):351-5. 10.1038/nature06090 PubMed 17721509