CBFRE-EGFP
(Plasmid #17705)

• Depositing Lab: Nicholas Gaiano
• Publication: Mizutani et al Nature. 2007 Sep 20. 449(7160):351-5.

Backbone

• Vector backbone: na
• Backbone size w/o insert (bp): 3600
• Vector type: Mammalian Expression, Adenoviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CBFRE
• Alt name: CBF1-responsive element
• Insert Size (bp): 1150
• Tag / Fusion Protein:
  • EGFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: SV40pro-F
• 3′ sequencing primer: EGFP-N

Resource Information

• Addgene Notes:
  • Addgene's diagnostic digest
• Articles Citing this Plasmid:
  • 9 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

CBFRE: four CBF1-binding elements from EBV (see Hsieh MCB 1996, 16:952) and the basal simian virus 40 (SV40) promoter upstream of EGFP. The sequence of the CBF1 binding element is GATCTGGAGTAACACGCCGTGGGAAAAAATTTATG. The binding site within this sequence is GTGGGAA.

A SmaI+XbaI double digest should yield 3.6kb and approx. 1.2kb bands. See Reviews link in right-hand column to view Addgene's diagnostic digest of this plasmid.