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Klotho (secreted)
(Plasmid #17713)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 17713 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA3.1/V5/His-TOPO
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5500
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Klotho
  • Species
    H. sapiens (human)
  • Entrez Gene
    KL (a.k.a. HFTC3)
  • Tags / Fusion Proteins
    • V5 (C terminal on insert)
    • His (C terminal on insert)

Cloning Information

Resource Information

Depositor Comments

The klotho gene expresses a secreted form, lacking KL2, transmembrane, and intracellular domains, due to alternative RNA splicing.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Klotho (secreted) was a gift from Hal Dietz (Addgene plasmid # 17713 ; http://n2t.net/addgene:17713 ; RRID:Addgene_17713)
  • For your References section:

    Association of human aging with a functional variant of klotho. Arking DE, Krebsova A, Macek M, Macek M, Arking A, Mian IS, Fried L, Hamosh A, Dey S, McIntosh I, Dietz HC. Proc Natl Acad Sci U S A. 2002 Jan 22. 99(2):856-61. 10.1073/pnas.022484299 PubMed 11792841