|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||17713||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5500
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Copy numberHigh Copy
SpeciesH. sapiens (human)
Entrez GeneKL (a.k.a. HFTC3)
/ Fusion Proteins
- V5 (C terminal on insert)
- His (C terminal on insert)
- Cloning method TOPO Cloning
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGH-rev (Common Sequencing Primers)
The klotho gene expresses a secreted form, lacking KL2, transmembrane, and intracellular domains, due to alternative RNA splicing.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:Klotho (secreted) was a gift from Hal Dietz (Addgene plasmid # 17713 ; http://n2t.net/addgene:17713 ; RRID:Addgene_17713)
For your References section:Association of human aging with a functional variant of klotho. Arking DE, Krebsova A, Macek M, Macek M, Arking A, Mian IS, Fried L, Hamosh A, Dey S, McIntosh I, Dietz HC. Proc Natl Acad Sci U S A. 2002 Jan 22. 99(2):856-61. 10.1073/pnas.022484299 PubMed 11792841