|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||17823||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Backbone manufacturerGroningen Biotechnology Center
Vector typeBacterial Expression, Synthetic Biology
Growth in Bacteria
Gene/Insert nameE. coli optimized red flourescent protein (RFPec2)
- Cloning method Restriction Enzyme
- 5′ sequencing primer n/a
- 3′ sequencing primer n/a (Common Sequencing Primers)
Please note that this plasmid contains GFP as well as RFP.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pBAD*RFPEC2 was a gift from Jay Keasling (Addgene plasmid # 17823 ; http://n2t.net/addgene:17823 ; RRID:Addgene_17823)
For your References section:Optimization of DsRed production in Escherichia coli: effect of ribosome binding site sequestration on translation efficiency. Pfleger BF, Fawzi NJ, Keasling JD. Biotechnol Bioeng. 2005 Dec 5. 92(5):553-8. 10.1002/bit.20630 PubMed 16247774