p415PGK1-AtMAX1(pYL759)
(Plasmid
#178289)
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PurposeExpresses MAX1 from A. thaliana (AtMAX1) in Saccharomyces cerevisiae. Centromeric LEU, PPGK1-AtMAX1-Tpho5
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 178289 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepAG415-ccdB
- Backbone size w/o insert (bp) 7667
- Total vector size (bp) 8572
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Vector typeYeast Expression
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Selectable markersLEU2
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameAtMAX1
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Alt nameMAX1 from A. thaliana
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SpeciesA. thaliana
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Insert Size (bp)1569
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GenBank IDNP_565617.2
- Promoter PGK1
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer M13 rev
- 3′ sequencing primer M13 for (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
p415PGK1-AtMAX1(pYL759) was a gift from Yanran Li (Addgene plasmid # 178289 ; http://n2t.net/addgene:178289 ; RRID:Addgene_178289) -
For your References section:
Establishment of strigolactone-producing bacterium-yeast consortium. Wu S, Ma X, Zhou A, Valenzuela A, Zhou K, Li Y. Sci Adv. 2021 Sep 17;7(38):eabh4048. doi: 10.1126/sciadv.abh4048. Epub 2021 Sep 17. 10.1126/sciadv.abh4048 PubMed 34533983
Map uploaded by the depositor.
