Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

Addgene
Establishment of strigolactone-producing bacterium-yeast consortium.
Wu S, Ma X, Zhou A, Valenzuela A, Zhou K, Li Y
Sci Adv. 2021 Sep 17;7(38):eabh4048. doi: 10.1126/sciadv.abh4048. Epub 2021 Sep 17. PubMed Article

Plasmids from Article

ID Plasmid Purpose
178285pCDFDuet-trAtCCD7-OsD27(pYL726 )Co-Expresses OsD27 and trAtCCD7 in E. coli. pCDFDuet-1 carrying D27 from Oryza sativa and N-terminus 31 amino-acid truncated CCD7 from Arabidopsis
178286pET21a-trAtCCD8(pYL735)Expresses trAtCCD8 in E. coli. pET21a carrying N-terminus 56 amino-acid truncated CCD8 from Arabidopsis
178287pET28b-trAtCCD8(pYL736)Expresses trAtCCD8 in E. coli. pET28b carrying t N-terminus 56 amino-acid truncated CCD8 from Arabidopsis
178288p413TEF1-ATR1(pYL573)Expresses NADPH-cytochrome P450 reductase 1 from A. thaliana (ATR1) in Saccharomyces cerevisiae. Centromeric HIS, PTEF1-ATR1-TCYC1
178289p415PGK1-AtMAX1(pYL759)Expresses MAX1 from A. thaliana (AtMAX1) in Saccharomyces cerevisiae. Centromeric LEU, PPGK1-AtMAX1-Tpho5
178290pAG414GPD-GaCYP722C(pYL777)Expresses GaCYP722C from G. arboreum in Saccharomyces cerevisiae. Centromeric TRP, PGPD-GaCYP722C-TCYC1
178291pAG414GPD-CaCYP722C(pYL803)Expresses CaCYP722C from C. annuum in Saccharomyces cerevisiae. Centromeric TRP, PGPD-CaCYP722C-TCYC1
178292pAG414GPD-LjCYP722C(pYL795)Expresses LjCYP722C from L. japonicus in Saccharomyces cerevisiae. Centromeric TRP, PGPD-LjCYP722C-TCYC1
178293pAG414GPD-VuCYP722C(pYL1070)Expresses VuCYP722C from V. unguiculata in Saccharomyces cerevisiae. Centromeric TRP, PGPD-VuCYP722C-TCYC1
178294pAG414GPD-SlCYP722C(pYL1071)Expresses SlCYP722C from S. lycopersicum in Saccharomyces cerevisiae. Centromeric TRP, PGPD-SlCYP722C-TCYC1
178295pAG414GPD-TpCYP722C(pYL851)Expresses TpCYP722C from T. pratense in Saccharomyces cerevisiae. Centromeric TRP, PGPD-TpCYP722C-TCYC1
178296pAG414GPD-CsCYP722C(pYL877)Expresses CsCYP722C from C. sinensis in Saccharomyces cerevisiae. Centromeric TRP, PGPD-CsCYP722C-TCYC1
178297pAG414GPD-VvCYP722C(pYL852)Expresses VvCYP722C from V. vinifera in Saccharomyces cerevisiae. Centromeric TRP, PGPD-VvCYP722C-TCYC1
178298pAG414GPD-GmCYP722C(pYL887)Expresses GmCYP722C from G. max in Saccharomyces cerevisiae. Centromeric TRP, PGPD-GmCYP722C-TCYC1
178299pAG414GPD-FvCYP722C2(pYL878)Expresses FvCYP722C2 from F. vesca in Saccharomyces cerevisiae. Centromeric TRP, PGPD-FvCYP722C2-TCYC1
178300pAG414GPD-OsCYP711A3(pYL761)Expresses OsCYP711A3 from O. sativa in Saccharomyces cerevisiae. Centromeric TRP, PGPD-OsCYP711A3-TCYC1
178301p416PGK1-OsCYP711A2(pYL770)Expresses OsCYP711A2 from O. sativa in Saccharomyces cerevisiae. Centromeric URA, PPGK1-OsCYP711A2-Tpho5
178302pAC-BETAipi-ispA/dxsProduces beta-carotene in E. coli; pAC-BETAipi inserted with an operon containing ispA and dxs from E. coli.

Antibodies from Article