Addgene - pCR4 hNFATc1-EE Plasmid Data

Plasmid 17857: pCR4 hNFATc1-EE

Gene/insert name:
NFATc1
Alt name:
NF-ATc
Insert size:
2200
Species:
H. sapiens (human)
Entrez Gene:
NFATC1 (NF-ATC, NFAT2, NFATc)
Fusion protein or tag:
EE
Terminal:
C terminal on insert
Vector backbone:
pCR4-TOPO
(Search Vector Database)
Backbone manufacturer:
Invitrogen
Vector type:
Bacterial Expression
Backbone size w/o insert (bp):
4000
Cloning method:
TOPO Cloning
5' sequencing primer:
T7 List of Sequencing Primers
3' sequencing primer:
T3
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (1)
Map:
View map
Principal Investigator:
Jerry Crabtree
Terms and Licenses:
MTA

Comments:

Full-length huNFATc1

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: NF-AT components define a family of transcription factors targeted in T-cell activation. Northrop et al (Nature. 1994 Jun 9. 369(6480):497-502. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 17857" in your Materials and Methods section.