pCR4-huNFATc1nuc
(Plasmid #23988)

• Depositing Lab: Jerry Crabtree
• Publication: Winslow et al Dev Cell. 2006 Jun . 10(6):771-82.

Backbone

• Vector backbone: pCR4
• Backbone size w/o insert (bp): 4000
• Vector type: cloning vector

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: NFATc1
• Alt name: NFATc1nuc
• Species: H. sapiens (human)
• Insert Size (bp): 2154
• Mutation: S172A S175A S176A S178A S179A S181A S184A S187A S188A S191A S194A S199A S203A S207A S211A S233A S237A S278A S282A S286A S290A
• Entrez Gene: NFATC1 (a.k.a. NF-ATC, NF-ATc1.2, NFAT2, NFATc)
• Tag / Fusion Protein:
  • EE tag (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamH1 (not destroyed)
• 5′ sequencing primer: T7 primer
• 3′ sequencing primer: T3 primer

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The c1nuc plasmid expresses the constitutively nuclear form of NFATc1 (Ho et al Nature 2004). It contains mutations in the phosphorylation sites for Dyrk1a and GSK3 that are responsible for actively exporting the protein from the nucleus (Beal et al Science 1997; Arron et al Nature 2006). It can be used as a transgene to explore NFAT signaling (Winslow et al Dev Cell 2006) and when expressed in a specific tissue can direct events such as bone development, thymocyte development or pancreatic beta cell functions (Heit J et al Nature 2007).