This website uses cookies to ensure you get the best experience. By continuing the use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

pCR4-huNFATc1nuc
(Plasmid #23988)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 23988 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NFATc1
  • Alt name
    NFATc1nuc
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2154
  • Mutation
    S172A S175A S176A S178A S179A S181A S184A S187A S188A S191A S194A S199A S203A S207A S211A S233A S237A S278A S282A S286A S290A
  • Entrez Gene
    NFATC1 (a.k.a. NF-ATC, NF-ATc1.2, NFAT2, NFATc)
  • Tag / Fusion Protein
    • EE tag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamH1 (not destroyed)
  • 5′ sequencing primer T7 primer
  • 3′ sequencing primer T3 primer
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses

Depositor Comments

The c1nuc plasmid expresses the constitutively nuclear form of NFATc1 (Ho et al Nature 2004). It contains mutations in the phosphorylation sites for Dyrk1a and GSK3 that are responsible for actively exporting the protein from the nucleus (Beal et al Science 1997; Arron et al Nature 2006). It can be used as a transgene to explore NFAT signaling (Winslow et al Dev Cell 2006) and when expressed in a specific tissue can direct events such as bone development, thymocyte development or pancreatic beta cell functions (Heit J et al Nature 2007).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCR4-huNFATc1nuc was a gift from Jerry Crabtree (Addgene plasmid # 23988)
  • For your References section:

    Calcineurin/NFAT signaling in osteoblasts regulates bone mass. Winslow MM, Pan M, Starbuck M, Gallo EM, Deng L, Karsenty G, Crabtree GR. Dev Cell. 2006 Jun . 10(6):771-82. 10.1016/j.devcel.2006.04.006 PubMed 16740479