Addgene - EGFPC1-huNFATc1EE-WT Plasmid Data

Plasmid 24219: EGFPC1-huNFATc1EE-WT

Gene/insert name:
NFATc1
Alt name:
NFAT2
Insert size:
2147
Species:
H. sapiens (human)
Entrez Gene:
NFATC1 (NF-ATC, NFAT2, NFATc)
Fusion protein or tag:
GFP
Terminal:
N terminal on backbone
Fusion protein or tag:
EE
Terminal:
C terminal on insert
Vector backbone:
pEGFPC1
(Search Vector Database)
Backbone manufacturer:
Clonetech
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
4700
Cloning site 5':
Bgl II
Site destroyed during cloning:
No
Cloning site 3':
BamH1
Site destroyed during cloning:
No
5' sequencing primer:
EGFP C seq. primer (#6478-1) List of Sequencing Primers
Bacterial resistance(s)
Kanamycin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (3)
Principal Investigator:
Jerry Crabtree
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Nuclear localization of NF-ATc by a calcineurin-dependent, cyclosporin-sensitive intramolecular interaction. Beals et al (Genes Dev. 1997 Apr 1. 11(7):824-34. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 24219" in your Materials and Methods section.