Addgene - pBJ5 mCnB Plasmid Data

Plasmid 17872: pBJ5 mCnB

Gene/insert name:
CnB
Alt name:
calcineurin B
Insert size:
600
Species:
M. musculus (mouse)
Entrez Gene:
Ppp3r1 (RP23-32H19.1, CaNB1, Cnb1, MCIP1)
Vector backbone:
pBJ5
(Search Vector Database)
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
3300
Cloning site 5':
SacII
Site destroyed during cloning:
No
Cloning site 3':
BamHI
Site destroyed during cloning:
No
5' sequencing primer:
pcDL-F List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (1)
Principal Investigator:
Jerry Crabtree
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Identification of calcineurin as a key signalling enzyme in T-lymphocyte activation. Clipstone et al (Nature. 1992 Jun 25. 357(6380):695-7. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 17872" in your Materials and Methods section.