Addgene - FLAG-mBAF45a IRES GFP Plasmid Data

Plasmid 17880: FLAG-mBAF45a IRES GFP

Gene/insert name:
BAF45a
Alt name:
Phf10
Insert size:
1226
Species:
M. musculus (mouse)
Entrez Gene:
Phf10 (AV024533, 1810055P05Rik)
Fusion protein or tag:
FLAG
Terminal:
N terminal on insert
Fusion protein or tag:
IRES EGFP
Terminal:
C terminal on backbone
Vector backbone:
Beta-actin/CMV-IRES-EGFP
(Search Vector Database)
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
6000
Cloning site 5':
KpnI
Site destroyed during cloning:
Yes
Cloning site 3':
BamHI
Site destroyed during cloning:
No
5' sequencing primer:
pCAG-F List of Sequencing Primers
3' sequencing primer:
IRES-R
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (2)
Map:
View map
Principal Investigator:
Jerry Crabtree
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: An essential switch in subunit composition of a chromatin remodeling complex during neural development. Lessard et al (Neuron. 2007 Jul 19. 55(2):201-15. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 17880" in your Materials and Methods section.