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FLAG-mBAF45a IRES GFP
(Plasmid #17880)

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 17880 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    Beta-actin/CMV-IRES-EGFP
  • Backbone size w/o insert (bp) 6000
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    BAF45a
  • Alt name
    Phf10
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1226
  • Entrez Gene
    Phf10 (a.k.a. 1810055P05Rik, AV024533)
  • Tags / Fusion Proteins
    • FLAG (N terminal on insert)
    • IRES EGFP (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (destroyed during cloning)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer pCAG-F
  • 3′ sequencing primer IRES-R
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    FLAG-mBAF45a IRES GFP was a gift from Jerry Crabtree (Addgene plasmid # 17880)
  • For your References section:

    An essential switch in subunit composition of a chromatin remodeling complex during neural development. Lessard J, Wu JI, Ranish JA, Wan M, Winslow MM, Staahl BT, Wu H, Aebersold R, Graef IA, Crabtree GR. Neuron. 2007 Jul 19. 55(2):201-15. 10.1016/j.neuron.2007.06.019 PubMed 17640523