pCAG-mcherry-WIPI2d-cs-TEV-STREP
(Plasmid #178912)

• Purpose: Mammalian expression of human WIPI2d with N-terminal mCherry and C-terminal Strep
• Depositing Lab: James Hurley
• Publication: Strong et al Elife. 2021 Sep 10;10. pii: 70372. doi: 10.7554/eLife.70372.

Backbone

• Vector backbone: pCAG
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: WIPI2d
• Species: H. sapiens (human)
• Tags / Fusion Proteins:
  • N term mcherry (N terminal on insert)
  • C term strep (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: ClaI (unknown if destroyed)
• 3′ cloning site: XmaI (unknown if destroyed)
• 5′ sequencing primer: pCAG for
• 3′ sequencing primer: pCAG Rev

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry