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Structural basis for membrane recruitment of ATG16L1 by WIPI2 in autophagy.
Strong LM, Chang C, Riley JF, Boecker CA, Flower TG, Buffalo CZ, Ren X, Stavoe AK, Holzbaur EL, Hurley JH
Elife. 2021 Sep 10;10. pii: 70372. doi: 10.7554/eLife.70372.
PubMed Article

Plasmids from Article

ID Plasmid Purpose
171830pCAG-WIPI2d10-364delta263-295-TEV-STREPMammalian expression of human WIPI2d 10-364 delta263-295 with C-terminal Strep
171831pCAG-WIPI2dH85E-cs-TEV-STREPMammalian expression of human WIPI2d H85E with C-terminal Strep
171832pCAG-WIPI2dK88E-cs-TEV-STREPMammalian expression of human WIPI2d K88E with C-terminal Strep
171833pCAG-WIPI2dI92E-cs-TEV-STREPMammalian expression of human WIPI2d I92E with C-terminal Strep
171834pCAG-WIPI2dC93E-cs-TEV-STREPMammalian expression of human WIPI2d C93E with C-terminal Strep
171835pCAG-WIPI2dR108E-cs-TEV-STREPMammalian expression of human WIPI2d R108E with C-terminal Strep
171836pCAG-WIPI2dR125E-cs-TEV-STREPMammalian expression of human WIPI2d R125E with C-terminal Strep
171837pCAG-WIPI2dK128E-cs-TEV-STREPMammalian expression of human WIPI2d K128E with C-terminal Strep
171838pCAG-mcherry-WIPI2dH85E-cs-TEV-STREPMammalian expression of human WIPI2d H85E with N-terminal mCherry and C-terminal Strep
171839pCAG-mcherry-WIPI2dK88E-cs-TEV-STREPMammalian expression of human WIPI2d K88E with N-terminal mCherry and C-terminal Strep
171840pCAG-mcherry-WIPI2dI92E-cs-TEV-STREPMammalian expression of human WIPI2d I92E with N-terminal mCherry and C-terminal Strep
171841pCAG-mcherry-WIPI2dC93E-cs-TEV-STREPMammalian expression of human WIPI2d C93E with N-terminal mCherry and C-terminal Strep
171842pCAG-mcherry-WIPI2dR108E-cs-TEV-STREPMammalian expression of human WIPI2d with N-terminal mCherry and C-terminal Strep
171843pCAG-mcherry-WIPI2dR125E-cs-TEV-STREPMammalian expression of human WIPI2d R108E with N-terminal mCherry and C-terminal Strep
171844pCAG-mcherry-WIPI2dK128E-cs-TEV-STREPMammalian expression of human WIPI2d K128E with N-terminal mCherry and C-terminal Strep
178912pCAG-mcherry-WIPI2d-cs-TEV-STREPMammalian expression of human WIPI2d with N-terminal mCherry and C-terminal Strep

Antibodies from Article