|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||17923||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector typeInsect Expression ; Drosophila metallothionein gene promoter drives expression
Growth in Bacteria
- Cloning method Restriction Enzyme
- 5′ sequencing primer MT forward (Common Sequencing Primers)
Insect expression vector.
This was the empty vector used in http://www.addgene.org/pubmed/17589500
This is NOT the vector by the same name described in the Castellino lab's 2008 Cytotechnology paper.
Notes from the Sabatini lab on using this plasmid:
They did a puromycin kill curve and ended up using 2 ug/mL of puromycin for selection for S2R cells. They used 5 mM CuSO4 to induce expression of their gene 24 hours before performing the IF experiments. The metallothionein promoter is described in this link: https://www.ncbi.nlm.nih.gov/pubmed/3125519
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pMT-puro was a gift from David Sabatini (Addgene plasmid # 17923 ; http://n2t.net/addgene:17923 ; RRID:Addgene_17923)