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Addgene

AAVS1-Puro Xlone SOX17
(Plasmid #179838)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 179838 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    Addgene #179837
  • Backbone size w/o insert (bp) 9654
  • Total vector size (bp) 10899
  • Vector type
    Mammalian Expression, AAV, CRISPR, TALEN, Synthetic Biology ; donor plasmid
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SOX17
  • Alt name
    VUR3
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1245
  • Entrez Gene
    SOX17 (a.k.a. VUR3)
  • Promoter TRE3GS

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site SpeI (not destroyed)
  • 5′ sequencing primer gcgcctataaaagagtgctga
  • 3′ sequencing primer cgcctgtcttaggttggagt
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Human SOX17 was cloned from Addgene plasmid #104541, a gift from David Vereide Lab.

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    AAVS1-Puro Xlone SOX17 was a gift from Xiaoping Bao (Addgene plasmid # 179838 ; http://n2t.net/addgene:179838 ; RRID:Addgene_179838)
  • For your References section:

    Temporal Expression of Transcription Factor ID2 Improves Natural Killer Cell Differentiation from Human Pluripotent Stem Cells. Jung J, Chang Y, Jin G, Lian X, Bao X. ACS Synth Biol. 2022 May 24. doi: 10.1021/acssynbio.2c00017. 10.1021/acssynbio.2c00017 PubMed 35608547