Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||18004||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4300
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Gene/Insert nameER targeted Bcl-2
Alt nameBcl-2 Cb5
Alt nameBcl2 Cytochrome b5
SpeciesH. sapiens (human)
Insert Size (bp)761
MutationC terminal of Bcl-2 replaced with Cytochrome b5. Targets Bcl-2 to the Endoplasmic Reticulum.
Entrez GeneBCL2 (a.k.a. Bcl-2, PPP1R50)
- Promoter CMV
/ Fusion Protein
- Flag (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
- 5′ sequencing primer T3
- 3′ sequencing primer T7 (Common Sequencing Primers)
Amino acids 100–134 (ITTIDSSSSWWTNWVIPAISAVAVALMYRLYMAED) human cytochrome b5 replaced the C-terminal domain of Bcl-2 (amino acids 219-239).
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:Flag-Bcl2-Cb5 was a gift from Clark Distelhorst (Addgene plasmid # 18004 ; http://n2t.net/addgene:18004 ; RRID:Addgene_18004)
For your References section:Transient expression of wild-type or mitochondrially targeted Bcl-2 induces apoptosis, whereas transient expression of endoplasmic reticulum-targeted Bcl-2 is protective against Bax-induced cell death. Wang NS, Unkila MT, Reineks EZ, Distelhorst CW. J Biol Chem. 2001 Nov 23. 276(47):44117-28. 10.1074/jbc.M101958200 PubMed 11546793