pRO16Sh2
(Plasmid #180604)

• Purpose: Expression of ShCAST under control of Plac with pRO1600 origin.
• Depositing Lab: Christopher Reisch
• Publication: Trujillo Rodriguez et al ACS Synth Biol. 2023 Jun 27. doi: 10.1021/acssynbio.3c00065.

Backbone

• Vector backbone: pR01600
• Backbone size w/o insert (bp): 3359
• Total vector size (bp): 9013
• Vector type: Bacterial Expression, Synthetic Biology

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: ShTnsB, ShTnsC, ShTniQ, ShCas12k
• Species: Scytonema hofmannii
• Insert Size (bp): 5988
• Promoter: pLac

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: GCTGGCCTTTTGCTCAACG
• 3′ sequencing primer: CACGACAGGTTTCCCGACTGGAAAGC

Resource Information

• Supplemental Documents:
  • pRO16sh2.gb
• A portion of this plasmid was derived from a plasmid made by: Addgene plasmid #127921 - pHelper_ShCAST_sgRNA

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The vector backbone was derived from plasmid pFlpe2 from

Choi, K. H., Mima, T., Casart, Y., Rholl, D., Kumar, A., Beacham, I. R., & Schweizer, H. P. (2008). Genetic tools for select-agent-compliant manipulation of Burkholderia pseudomallei. Applied and environmental microbiology, 74(4), 1064–1075. https://doi.org/10.1128/AEM.02430-07