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CRISPR-Associated Transposase for Targeted Mutagenesis in Diverse Proteobacteria.
Trujillo Rodriguez L, Ellington AJ, Reisch CR, Chevrette MG
ACS Synth Biol. 2023 Jun 27. doi: 10.1021/acssynbio.3c00065. PubMed Article

Plasmids from Article

ID Plasmid Purpose
170981pBLlSh2Expression of ShCAST under control of lacI Plac promoter/repressor pair.
170982pUCSh2-mRFPEncodes sgRNA scaffold with mRFP in place of target and Kanamycin resistance transposon. New targets can be cloned replacing mRFP using around the horn cloning.
170983pUCSh3-mRFPEncodes sgRNA scaffold with mRFP in place of target and Tetracycline resistance transposon. New targets can be cloned replacing mRFP using around the horn cloning.
170984pUCSh2-2300Encodes sgRNA target 2300 and Kanamycin resistance transposon.
170985pUCSh3-2300Encodes sgRNA target 2300 and Tetracycline resistance transposon.
170986pUCSh8-2300Encodes sgRNA target 2300 and Hygromycin resistance transposon.
170987pUCSh5vio-2300Encodes sgRNA target 2300 and Gentamicin resistance - violacein pathway transposon.
171788pUCSh5-2300Encodes sgRNA target 2300 and Gentamicin resistance transposon.
180076pRO16Sh5Expression of ShCAST under control of Plac with pRO1600 origin.
180604pRO16Sh2Expression of ShCAST under control of Plac with pRO1600 origin.

Antibodies from Article