pAAV-shMcu-1
(Plasmid #181868)

• Purpose: Expresses Mcu-targeted shRNA under control of the U6 promoter and hrGFP under control of the synthetic CAG promoter
• Depositing Lab: Hilmar Bading
• Publication: Qiu et al Nat Commun. 2013;4:2034. doi: 10.1038/ncomms3034.

Backbone

• Vector backbone: pAAV-U6_CAG-hrGFP
• Vector type: Mammalian Expression, Mouse Targeting, Adenoviral, AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: mitochondrial calcium uniporter
• Alt name: MCU
• gRNA/shRNA sequence: TAGGGAATAAAGGGATCTTA
• Species: M. musculus (mouse)
• GenBank ID: NM_001033259.4
• Entrez Gene: Mcu (a.k.a. 2010012O16Rik, C10orf42, Ccdc109a, D130073L02Rik, Gm64)
• Promoter: U6
• Tag / Fusion Protein:
  • hrGFP (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: TTGGGTAGTTTGCAGTTTTAAAATT
• 3′ sequencing primer: TCACTGAGGAAGCTCTCTG

Resource Information

• Supplemental Documents:
  • U6-sh(MCU1)_CAG-GFP.gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry