pmiRGLO Dual-Luciferase-MYB-3'UTR
(Plasmid #182250)

• Purpose: Human MYB 3` UTR region containing 3x binding sites for miR-150 cloned downstream of luciferase reporter gene
• Depositing Lab: Silvia Monticelli
• Publication: Chirichella et al PLoS Biol. 2022 Feb 10;20(2):e3001538. doi: 10.1371/journal.pbio.3001538.

Backbone

• Vector backbone: pmirGLO Dual-Luciferase
• Backbone manufacturer: Promega
• Backbone size w/o insert (bp): 7344
• Total vector size (bp): 7876
• Vector type: Mammalian Expression, Luciferase

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: 3' UTR region of MYB gene
• Species: H. sapiens (human)
• Insert Size (bp): 532

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (unknown if destroyed)
• 3′ cloning site: XbaI (unknown if destroyed)
• 5′ sequencing primer: AGA AGC TGC GCG GTG GTG TTG TG

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
  • Renilla Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry