Addgene - BA-Wnt10b Plasmid Data

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1831: BA-Wnt10b
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1821: neo/TAP Myc
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Plasmid 1831: BA-Wnt10b

Gene/insert name:
Wnt10b
Alt name:
Wnt-10b
Alt name:
wingless related MMTV integration site 10b
Alt name:
beta-actin promoter
Insert size:
3400
Species:
M. musculus (mouse)
Entrez Gene:
Wnt10b (Wnt12)
Vector backbone:
pCAGGS
(Search Vector Database)
Vector type:
Mammalian Expression
Backbone size w/o insert:
3200
Cloning site 5':
EcoRI
Site destroyed during cloning:
No
Cloning site 3':
EcoRI
Site destroyed during cloning:
No
5' sequencing primer:
na List of Sequencing Primers
3' sequencing primer:
Bglob-pA-R
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (1)
Map:
View map
Principal Investigator:
Philip Leder
Terms and Licenses:
MTA

Comments:

See scanned map.
(Leder #C-893)

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Wnt-10b directs hypermorphic development and transformation in mammary glands of male and female mice. Lane et al (Oncogene 1997 Oct;15(18):2133-44. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 1831" in your Materials and Methods section.