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pCS2-hDKK1-flag
(Plasmid #15494)

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 15494 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    DKK1
  • Alt name
    Dickkopf-1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    830
  • GenBank ID
    AF177394
  • Entrez Gene
    DKK1 (a.k.a. DKK-1, SK)
  • Tag / Fusion Protein
    • flag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer SP6
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Articles Citing this Plasmid

Depositor Comments

See author's map for details.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCS2-hDKK1-flag was a gift from Sergei Sokol (Addgene plasmid # 15494 ; http://n2t.net/addgene:15494 ; RRID:Addgene_15494)
  • For your References section:

    Functional and structural diversity of the human Dickkopf gene family. Krupnik VE, Sharp JD, Jiang C, Robison K, Chickering TW, Amaravadi L, Brown DE, Guyot D, Mays G, Leiby K, Chang B, Duong T, Goodearl AD, Gearing DP, Sokol SY, McCarthy SA. Gene. 1999 Oct 1. 238(2):301-13. 10.1016/S0378-1119(99)00365-0 PubMed 10570958