|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||15494||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4100
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
SpeciesH. sapiens (human)
Insert Size (bp)830
Entrez GeneDKK1 (a.k.a. DKK-1, SK)
/ Fusion Protein
- flag (C terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer SP6 (Common Sequencing Primers)
See author's map for details.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCS2-hDKK1-flag was a gift from Sergei Sokol (Addgene plasmid # 15494 ; http://n2t.net/addgene:15494 ; RRID:Addgene_15494)
For your References section:Functional and structural diversity of the human Dickkopf gene family. Krupnik VE, Sharp JD, Jiang C, Robison K, Chickering TW, Amaravadi L, Brown DE, Guyot D, Mays G, Leiby K, Chang B, Duong T, Goodearl AD, Gearing DP, Sokol SY, McCarthy SA. Gene. 1999 Oct 1. 238(2):301-13. 10.1016/S0378-1119(99)00365-0 PubMed 10570958
Map uploaded by the depositor.