Addgene - pRS414 GPD-Sis1deltaG/F Plasmid Data

Plasmid 18688: pRS414 GPD-Sis1deltaG/F

Gene/insert name:
Sis1 deltaG/F
Alt name:
Sis1 dG/F
Alt name:
GPD-Sis1 dG/F p414
Insert size:
908
Species:
S. cerevisiae (budding yeast)
GenBank ID:
X58460
Mutation:
Sis1 with the G/F region deleted
Vector backbone:
pRS414 GPD
(Search Vector Database)
Vector type:
Yeast Expression
Backbone size w/o insert (bp):
5665
Cloning site 5':
Spe1
Site destroyed during cloning:
No
Cloning site 3':
BamH1
Site destroyed during cloning:
No
5' sequencing primer:
ATGGTCAAGGAGACAAAAC List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
Low Copy
Selectable markers:
TRP1
Person or lab that originally
cloned the gene/insert:
This gene was originally cloned by Elizabeth Craig's lab. Sis1dG/F was amplified from one of their plasmids and cloned in the pRS414 GPD plasmid.
Sequence: View sequences (1)
Map:
View map
Principal Investigator:
Susan Lindquist
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Chaperone-dependent amyloid assembly protects cells from prion toxicity. Douglas et al (Proc Natl Acad Sci U S A. 2008 May 20. 105(20):7206-11. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 18688" in your Materials and Methods section.