pRS414 GPD-Sis1deltaG/F
(Plasmid #18688)

Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    pRS414 GPD
  • Backbone size w/o insert (bp) 5665
  • Vector type
    Yeast Expression
  • Selectable markers
    TRP1

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Sequence Information

Full plasmid sequence is available only if provided by the depositing laboratory.

Gene/Insert

  • Gene/Insert name
    Sis1 deltaG/F
  • Alt name
    Sis1 dG/F
  • Alt name
    GPD-Sis1 dG/F p414
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    908
  • Mutation
    Sis1 with the G/F region deleted
  • GenBank ID
    X58460

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Spe1 (not destroyed)
  • 3′ cloning site BamH1 (not destroyed)
  • 5′ sequencing primer ATGGTCAAGGAGACAAAAC
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    This gene was originally cloned by Elizabeth Craig's lab. Sis1dG/F was amplified from one of their plasmids and cloned in the pRS414 GPD plasmid.
  • Terms and Licenses
How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRS414 GPD-Sis1deltaG/F was a gift from Susan Lindquist (Addgene plasmid # 18688)
  • For your References section:

    Chaperone-dependent amyloid assembly protects cells from prion toxicity. Douglas PM, Treusch S, Ren HY, Halfmann R, Duennwald ML, Lindquist S, Cyr DM. Proc Natl Acad Sci U S A. 2008 May 20. 105(20):7206-11. 10.1073/pnas.0802593105 PubMed 18480252