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Plasmid 18688: pRS414 GPD-Sis1deltaG/F
  • Sis1 deltaG/F

  • Sis1 dG/F

  • GPD-Sis1 dG/F p414

  • 908

  • S. cerevisiae (budding yeast)

  • X58460

  • Sis1 with the G/F region deleted

  • pRS414 GPD
    (Search Vector Database)

  • Yeast Expression

  • 5665

  • Spe1

  • No

  • BamH1

  • No

  • ATGGTCAAGGAGACAAAAC List of Sequencing Primers

  • Ampicillin

  • DH5alpha

  • 37

  • Low Copy

  • TRP1

  • This gene was originally cloned by Elizabeth Craig's lab. Sis1dG/F was amplified from one of their plasmids and cloned in the pRS414 GPD plasmid.

  • View sequences (1)
  • View map

  • Susan Lindquist

  • MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Chaperone-dependent amyloid assembly protects cells from prion toxicity. Douglas et al (Proc Natl Acad Sci U S A. 2008 May 20. 105(20):7206-11. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 18688" in your Materials and Methods section.