pRRL-5xTetO-pEF-H2B-Citrine-SV40
(Plasmid #186966)

• Purpose: Lentiviral vector encoding fluorescent reporter (5xTetO-pEF-H2B-Citrine-SV40) for expression in mammalian cells.
• Depositing Lab: Brian Liau
• Publication: Lue et al Nat Chem Biol. 2023 Feb;19(2):176-186. doi: 10.1038/s41589-022-01167-4. Epub 2022 Oct 20.

Backbone

• Vector backbone: pRRL
• Backbone size w/o insert (bp): 5474
• Total vector size (bp): 8633
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Citrine (fluorescence)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: 5xTetO-pEF-H2B-Citrine-SV40
• Species: H. sapiens (human), Synthetic
• Insert Size (bp): 3211
• Promoter: 5xTetO-pEF-1alpha

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XbaI (not destroyed)
• 3′ cloning site: KpnI (not destroyed)
• 5′ sequencing primer: cPPT-SF ATAGTAGACATAATAGCAACAGACATAC
• 3′ sequencing primer: Citrine-SF CATGGTCCTGCTGGAGTTCGTG

Resource Information

• Supplemental Documents:
  • pRRL-5xTetO-pEF-H2B-Citrine-SV40.gb
• A portion of this plasmid was derived from a plasmid made by: The 5xTetO-pEF-H2B-Citrine-SV40 insert was amplified from PhiC31-Neo-ins-5xTetO-pEF-H2B-Citrine-ins, a gift from M. Elowitz (Addgene #78099). The pRRL vector backbone was prepared by restriction digest of LT3REVIR, a gift from J. Zuber (Addgene #111176).

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry