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(Plasmid #18949)

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Available to Academic and Nonprofits Only


  • Vector backbone
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 5433
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Growth instructions
  • Copy number
    High Copy

Sequence Information


  • Gene/Insert name
    Hypoxia inducible factor 1 alpha
  • Alt name
  • Species
    H. sapiens (human)
  • Insert Size (bp)
  • Mutation
    wt cDNA
  • GenBank ID
  • Entrez Gene
    HIF1A (a.k.a. HIF-1-alpha, HIF-1A, HIF-1alpha, HIF1, HIF1-ALPHA, MOP1, PASD8, bHLHe78)
  • Tag / Fusion Protein
    • HA-tag (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamH I (not destroyed)
  • 3′ cloning site EcoR I (destroyed during cloning)
  • 5′ sequencing primer T7
  • 3′ sequencing primer SP6
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    HA-HIF1alpha-pcDNA3 was a gift from William Kaelin (Addgene plasmid # 18949)
  • For your References section:

    Inhibition of HIF is necessary for tumor suppression by the von Hippel-Lindau protein. Kondo K, Klco J, Nakamura E, Lechpammer M, Kaelin WG. Cancer Cell. 2002 Apr . 1(3):237-46. 10.1016/S1535-6108(02)00043-0 PubMed 12086860