|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||18949||Plasmid sent as bacteria in agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5433
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameHypoxia inducible factor 1 alpha
SpeciesH. sapiens (human)
Insert Size (bp)2505
Entrez GeneHIF1A (a.k.a. HIF-1-alpha, HIF-1A, HIF-1alpha, HIF1, HIF1-ALPHA, MOP1, PASD8, bHLHe78)
/ Fusion Protein
- HA-tag (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site BamH I (not destroyed)
- 3′ cloning site EcoR I (destroyed during cloning)
- 5′ sequencing primer T7
- 3′ sequencing primer SP6 (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:HA-HIF1alpha-pcDNA3 was a gift from William Kaelin (Addgene plasmid # 18949)
For your References section:Inhibition of HIF is necessary for tumor suppression by the von Hippel-Lindau protein. Kondo K, Klco J, Nakamura E, Lechpammer M, Kaelin WG. Cancer Cell. 2002 Apr . 1(3):237-46. 10.1016/S1535-6108(02)00043-0 PubMed 12086860
Generated by Addgene from full sequence supplied by depositor.
Map uploaded by the depositor.