Addgene - HA-HIF1alpha P402A/P564A-pcDNA3 Plasmid Data

Plasmid 18955: HA-HIF1alpha P402A/P564A-pcDNA3

Gene/insert name:
Hypoxia inducible factor 1 alpha
Alt name:
HIF1a
Insert size:
2505
Species:
H. sapiens (human)
GenBank ID:
NM_001530
Entrez Gene:
HIF1A (HIF-1A, HIF-1alpha, HIF1, HIF1-ALPHA, MOP1, PASD8, bHLHe78)
Fusion protein or tag:
HA-tag
Terminal:
N terminal on backbone
Mutation:
cDNA changed amino acids: Proline 402 to Alanine Proline 564 to Alanine
Vector backbone:
pcDNA3
(Search Vector Database)
Backbone manufacturer:
Invitrogen
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
5433
Cloning site 5':
BamH I
Site destroyed during cloning:
No
Cloning site 3':
EcoR I
Site destroyed during cloning:
Yes
5' sequencing primer:
T7 List of Sequencing Primers
3' sequencing primer:
SP6
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
Growth instructions:
DH5alpha
High or low copy:
High Copy
Selectable markers:
Neomycin
Sequence: View sequences (4)
Map:
View map
Principal Investigator:
William Kaelin
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: The hypoxia-inducible factor 2alpha N-terminal and C-terminal transactivation domains cooperate to promote renal tumorigenesis in vivo. Yan et al (Mol Cell Biol. 2007 Mar . 27(6):2092-102. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 18955" in your Materials and Methods section.