pTR1-GPD-TRPC
(Plasmid
#190103)
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Purpose(Empty Backbone) Expression vector for fungal (Aspergillus flavus) genes based on pyrithiamine resistance (ptrA) selection
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 190103 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepPTR1
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Backbone manufacturerTaKaRa
- Backbone size (bp) 4782
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Modifications to backboneInserted Aspergillus nidulans gpdA promoter and trpC terminator with unique NotI and SmaI sites for cloning (Ping Li & P-K Chang)
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Vector typeYeast Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Cloning Information
- Cloning method Restriction Enzyme
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byOriginally constructed by Ping Li (USDA, ARS, Southern Regional Research Center) and modified by P-K Chang
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pTR1-GPD-TRPC was a gift from Perng-Kuang Chang (Addgene plasmid # 190103 ; http://n2t.net/addgene:190103 ; RRID:Addgene_190103) -
For your References section:
Deletion of the Aspergillus flavus orthologue of A. nidulans fluG reduces conidiation and promotes production of sclerotia but does not abolish aflatoxin biosynthesis. Chang PK, Scharfenstein LL, Mack B, Ehrlich KC. Appl Environ Microbiol. 2012 Nov;78(21):7557-63. doi: 10.1128/AEM.01241-12. Epub 2012 Aug 17. 10.1128/AEM.01241-12 PubMed 22904054
