CD23b in pIRES2eGFP
(Plasmid #19136)

• Depositing Lab: Zena Werb
• Publication: Lemieux et al J Biol Chem. 2007 May 18. 282(20):14836-44.

Backbone

• Vector backbone: pIRES2eGFP
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 5300
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CD23 beta
• Alt name: CD23b
• Species: H. sapiens (human)
• Insert Size (bp): 993
• GenBank ID: NM_002002
• Entrez Gene: FCER2 (a.k.a. BLAST-2, CD23, CD23A, CLEC4J, FCE2, FCErII, IGEBF)
• Tag / Fusion Protein:
  • HA (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: n/a
• 3′ sequencing primer: n/a

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry