|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||19158||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4657
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameTransforming growth factor, beta receptor II
Alt nameAAT3; FAA3; MFS2; RIIC; LDS1B; LDS2B; TAAD2; TGFR-2; TGFbeta-RII
SpeciesH. sapiens (human)
Insert Size (bp)27
MutationPCR based mutagenesis
Entrez GeneTGFBR2 (a.k.a. AAT3, FAA3, LDS1B, LDS2, LDS2B, MFS2, RIIC, TAAD2, TBR-ii, TBRII, TGFR-2, TGFbeta-RII)
/ Fusion Protein
- HA (YDVPDYASL) (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site SalI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer CMV Forward (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made byJacqueline Doody
Terms and Licenses
See author map for cloning details.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCMV5 HA-TGFBRII was a gift from Joan Massague (Addgene plasmid # 19158 ; http://n2t.net/addgene:19158 ; RRID:Addgene_19158)
Map uploaded by the depositor.