pSpCas9_T2A_iRFP670 sgBFP
(Plasmid
#192478)
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PurposeAll-in-one plasmid for cutting BFP region in DSB Spectrum V2 or V3
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 192478 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepX459
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Backbone manufacturerAddgene Plasmid #62988
- Total vector size (bp) 9501
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Modifications to backboneReplaced Puromycin gene with iRFP670 fluorescent protein and inserted BFP sgRNA
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSpCas9
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Tag
/ Fusion Protein
- T2A iRFP670 (C terminal on insert)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSpCas9_T2A_iRFP670 sgBFP was a gift from Michael Yaffe (Addgene plasmid # 192478 ; http://n2t.net/addgene:192478 ; RRID:Addgene_192478) -
For your References section:
Multi-pathway DNA-repair reporters reveal competition between end-joining, single-strand annealing and homologous recombination at Cas9-induced DNA double-strand breaks. van de Kooij B, Kruswick A, van Attikum H, Yaffe MB. Nat Commun. 2022 Sep 8;13(1):5295. doi: 10.1038/s41467-022-32743-w. 10.1038/s41467-022-32743-w PubMed 36075911
