Multi-pathway DNA-repair reporters reveal competition between end-joining, single-strand annealing and homologous recombination at Cas9-induced DNA double-strand breaks.
van de Kooij B, Kruswick A, van Attikum H, Yaffe MB
Nat Commun. 2022 Sep 8;13(1):5295. doi: 10.1038/s41467-022-32743-w. PubMed Article
van de Kooij B, Kruswick A, van Attikum H, Yaffe MB
Nat Commun. 2022 Sep 8;13(1):5295. doi: 10.1038/s41467-022-32743-w. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 192474 | pLVX_DSB_Spectrum-V1 | Lentiviral DSB Spectrum V1 Reporter System |
| 192475 | pLVX_DSB_Spectrum-V2 | Lentiviral DSB Spectrum V2 Reporter System |
| 192476 | pLVX_DSB_Spectrum-V3 | Lentiviral DSB Spectrum V3 Reporter System |
| 192477 | pSpCas9_T2A_iRFP670 sgAAVS1 | All-in-one plasmid for cutting non-targeting locus in DSB Spectrum V1, V2, or V3 cells as a control |
| 192478 | pSpCas9_T2A_iRFP670 sgBFP | All-in-one plasmid for cutting BFP region in DSB Spectrum V2 or V3 |
| 192479 | pSpCas9_T2A_mCherry sg1-sg2 | All-in-one plasmid containing both guides for cutting BFP region in DSB Spectrum V1 |
| 192480 | pSpCas9_T2A_mCherry sgAAVS1 | All-in-one plasmid for cutting non-targeting locus in DSB Spectrum V1 or V2 cells as control |
| 192929 | pSpCas9_T2A_mCherry sgBFP | All-in-one plasmid for cutting BFP region in DSB Spectrum V2 |