Flag pLJM1 Rheb1
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||19312||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 7400
Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
Growth instructionsGrow in Exel10Gold or Stbl2 cells
Copy numberHigh Copy
Gene/Insert nameRHEB Ras homolog enriched in brain
SpeciesH. sapiens (human)
Insert Size (bp)560
Entrez GeneRHEB (a.k.a. RHEB2)
/ Fusion Protein
- Flag (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site SalI (not destroyed)
- 3′ cloning site EcoRV (not destroyed)
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
pLJM1 is based on pLKO.1. pLJM1 was cut with AgeI and EcoRI and a Flag tag was cloned in. The cDNA was cloned into Flag-pLJM1 with SalI and EcoRV. The sequence across the MCS includes AgeI-Flag-SalI-gene-EcoRV-EcoRI
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:Flag pLJM1 Rheb1 was a gift from David Sabatini (Addgene plasmid # 19312 ; http://n2t.net/addgene:19312 ; RRID:Addgene_19312)
For your References section:The Rag GTPases bind raptor and mediate amino acid signaling to mTORC1. Sancak Y, Peterson TR, Shaul YD, Lindquist RA, Thoreen CC, Bar-Peled L, Sabatini DM. Science. 2008 Jun 13. 320(5882):1496-501. 10.1126/science.1157535 PubMed 18497260