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Plasmid 19317: Flag pLJM1 RagD 77L
  • Ras-related GTP binding D

  • RRAGD

  • 1200

  • H. sapiens (human)

  • RRAGD (RAGD, bA11D8.2.1)

  • Flag

  • N terminal on insert

  • S77L

  • pLJM1
    (Search Vector Database)

  • Mammalian Expression, Lentiviral

  • 7400

  • SalI

  • No

  • EcoRV

  • No

  • CMV-F List of Sequencing Primers

  • Ampicillin

  • Stbl3

  • 37

  • Grow in Exel10Gold or Stbl2 cells

  • High Copy

  • Puromycin

  • View sequences (2)
  • David Sabatini

  • UBMTA

Comments: 

pLJM1 is based on pLKO.1. pLJM1 was cut with AgeI and EcoRI and a Flag tag was cloned in. The cDNA was cloned into Flag-pLJM1 with SalI and EcoRV. The sequence across the MCS includes AgeI-Flag-SalI-gene-EcoRV-EcoRI

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: The Rag GTPases bind raptor and mediate amino acid signaling to mTORC1. Sancak et al (Science. 2008 Jun 13. 320(5882):1496-501. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 19317" in your Materials and Methods section.