GFP-RAPH1ΔTBS
(Plasmid #194859)

• Purpose: Express eGFP-RAPH1 lacking the TBS domain (RAPH1 aa 2-92 deleted) in mammalian cells.
• Depositing Lab: Guillaume Jacquemet
• Publication: Popovic et al J Cell Sci. 2023 Mar 1;136(5):jcs260574. doi: 10.1242/jcs.260574. Epub 2023 Mar 2.

Backbone

• Vector backbone: EGFPC1
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: RAPH1
• Alt name: Lamellipodin
• Species: H. sapiens (human)
• Mutation: RAPH1 with amino acids 2-92 deleted
• Entrez Gene: RAPH1 (a.k.a. ALS2CR18, ALS2CR9, LPD, PREL-2, PREL2, RMO1, RalGDS/AF-6)
• Promoter: CMV
• Tag / Fusion Protein:
  • eGFP (N terminal on backbone)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: EGFP-C

Resource Information

• A portion of this plasmid was derived from a plasmid made by: The original EGFP-Lpd plasmid was kindly provided by Matthias Krause (King’s College London).

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The GFP-RAPH1ΔTBS (RAPH1 aa 2-92 deleted) construct was created by inserting a custom gene block (IDT) in the EGFP-Lpd plasmid using the XhoI/HindIII sites.