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Myosin-X recruits lamellipodin to filopodia tips.
Popovic A, Miihkinen M, Ghimire S, Saup R, Gronloh MLB, Ball NJ, Goult BT, Ivaska J, Jacquemet G
J Cell Sci. 2023 Mar 1;136(5):jcs260574. doi: 10.1242/jcs.260574. Epub 2023 Mar 2. PubMed Article

Plasmids from Article

ID Plasmid Purpose
194858GFP-MYO10-BioIDExpress MYO10 tagged with eGFP (Nter) and BioID (Cter) in mammalian cells.
194859GFP-RAPH1ΔTBSExpress eGFP-RAPH1 lacking the TBS domain (RAPH1 aa 2-92 deleted) in mammalian cells.
194860eGFP-RAPH1-F1Express the RAPH1 fragment (1-535) tagged to eGFP in mammalian cells.
194861eGFP-RAPH1-F2Express the RAPH1 fragment (535-868) tagged to eGFP in mammalian cells.
194862eGFP-RAPH1-F3Express the RAPH1 fragment (868-1062) tagged to eGFP in mammalian cells.
194863eGFP-RAPH1-F4Express the RAPH1 fragment (1062-1250) tagged to eGFP in mammalian cells.
194864GST-RAPH1-F2Express the RAPH1 fragment (535-868) tagged to GST in bacteria.
194865GFP-RAPH1Δ536-587Express eGFP-RAPH1 lacking amino acids 536-587 in mammalian cells. This constructs does not localise to filopodia anymore.

Antibodies from Article