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pcDNA5/FRT/TO HIS HSPA1A
(Plasmid #19537)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 19537 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA5/FRT/TO
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5137
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    heat shock 70kDa protein 1A
  • Alt name
    HSPA1A
  • Alt name
    HSP72; HSPA1; HSP70I; HSPA1B; HSP70-1; HSP70-1A
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1955
  • Entrez Gene
    HSPA1A (a.k.a. HEL-S-103, HSP70-1, HSP70-1A, HSP70-2, HSP70.1, HSP70.2, HSP70I, HSP72, HSPA1)
  • Tag / Fusion Protein
    • His (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer LNCX
  • 3′ sequencing primer BGH-rev
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

Part of the HSP70 gene family

Disclaimer:

For cloning the various HSP genes, the Stratagene reference RNA for cDNA synthesis was used and genes were amplified from this mixture that originates from 10 different cell lines from different somatic origin and ultimately coming from 10 different individuals. This strategy was used to maximize the chance for the presence of individual HSP transcripts. Users must be aware that this mixture thus represents 20 complete haploid genomes, meaning that for each individual gene there might be polymorphisms that could potentially have functional consequences. The library was sequence verified only for the presence of the correct gene.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA5/FRT/TO HIS HSPA1A was a gift from Harm Kampinga (Addgene plasmid # 19537 ; http://n2t.net/addgene:19537 ; RRID:Addgene_19537)
  • For your References section:

    Computational analysis of the human HSPH/HSPA/DNAJ family and cloning of a human HSPH/HSPA/DNAJ expression library. Hageman J, Kampinga HH. Cell Stress Chaperones. 2008 Aug 7. ():. 10.1007/s12192-008-0060-2 PubMed 18686016