pFastBac1 Flag BRG1
(Plasmid #1957)

• Depositing Lab: Robert Kingston
• Publication: Phelan et al Mol Cell 1999 Feb;3(2):247-53.

Backbone

• Vector backbone: pFastBac1
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 4775
• Vector type: Insect Expression
• Selectable markers: Gentamicin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: BRG1
• Alt name: BAF190a
• Species: H. sapiens (human)
• Entrez Gene: SMARCA4 (a.k.a. BAF190, BAF190A, BRG1, CSS4, MRD16, RTPS2, SNF2, SNF2-beta, SNF2L4, SNF2LB, SWI2, hSNF2b)
• Tag / Fusion Protein:
  • flag (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SalI (not destroyed)
• 3′ cloning site: SalI (not destroyed)
• 5′ sequencing primer: Polyhedrin forward

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

A C-terminal Flag tag was added to BRG1 by PCR using a 3? primer designed to incorporate the FLAG sequence (DYKDDDDK) before the stop codon, followed by SalI and SpeI restriction sites. The PCR product was digested with BglII and SpeI and cloned into pBS/Brg1. The full-length Brg1 cDNA was then excised out of pBS/FL-Brg1 as a SalI fragment and cloned into pFastBac1 to generate pFastBac1/Brg1(Flag). (kingston #1306)