pFSynW SYT9 D330, 332N IRES GFP
(Plasmid #195702)

• Purpose: Lentiviral plasmid encoding SYT9 with D330, 332N mutations followed by an internal ribosomal entry site followed by EGFP under the human synapsin promoter
• Depositing Lab: Edwin Chapman
• Publication: Seibert et al J Neurosci. 2023 Mar 1;43(9):1475-1491. doi: 10.1523/JNEUROSCI.1857-22.2023. Epub 2023 Feb 2.

Backbone

• Vector backbone: FUGW
• Backbone manufacturer: David Baltimore (Addgene plasmid # 14883)
• Vector type: Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Syt9
• Species: M. musculus (mouse)
• Mutation: full-length mouse SYT9 with D330, 332N mutations
• Entrez Gene: Syt5 (a.k.a. SytV)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Unknown (unknown if destroyed)
• 3′ cloning site: Unknown (unknown if destroyed)
• 5′ sequencing primer: n/a

Resource Information

• Supplemental Documents:
  • pFSynW SYT9 D330, 332N IRES GFP.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

full-length mouse SYT9 with D330, 332N mutations; bicistronic EGFP expression

Please visit https://www.biorxiv.org/content/10.1101/2022.04.18.488681v2 for bioRxiv preprint.