Synaptotagmin 9 Modulates Spontaneous Neurotransmitter Release in Striatal Neurons by Regulating Substance P Secretion.
Seibert MJ, Evans CS, Stanley KS, Wu Z, Chapman ER
J Neurosci. 2023 Mar 1;43(9):1475-1491. doi: 10.1523/JNEUROSCI.1857-22.2023. Epub 2023 Feb 2. PubMed Article
Seibert MJ, Evans CS, Stanley KS, Wu Z, Chapman ER
J Neurosci. 2023 Mar 1;43(9):1475-1491. doi: 10.1523/JNEUROSCI.1857-22.2023. Epub 2023 Feb 2. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 195698 | pFSynW pHluorin-SYT9 IRES mRuby3 | Lentiviral plasmid encoding SYT9 with an N-terminal pHluorin tag followed by an internal ribosomal entry site followed by mRuby3 under the human synapsin promoter |
| 195699 | pFSynW Substance P-pHluorin IRES mRuby3 | Lentiviral plasmid encoding first 68 residues of mouse preprotachykinin with a C-terminal pHluorin tag followed by an internal ribosomal entry site followed by mRuby3 under the human synapsin promoter |
| 195700 | pFSynW Syt9 IRES mRuby3 | Lentiviral plasmid encoding full-length mouse SYT9 followed by an internal ribosomal entry site followed by mRuby under the human synapsin promoter |
| 195701 | pFSynW SYT9 D197, 199N IRES GFP | Lentiviral plasmid encoding SYT9 with D197, 199N mutations followed by an internal ribosomal entry site followed by EGFP under the human synapsin promoter |
| 195702 | pFSynW SYT9 D330, 332N IRES GFP | Lentiviral plasmid encoding SYT9 with D330, 332N mutations followed by an internal ribosomal entry site followed by EGFP under the human synapsin promoter |
| 195703 | pFSynW SYT9 D197, 199, 330, 332N IRES GFP | Lentiviral plasmid encoding SYT9 with D197, 199, 330, 332N mutations followed by an internal ribosomal entry site followed by EGFP under the human synapsin promoter |
| 195704 | pGEX SYT9 C2A D197,199N | Bacterial expression plasmid encoding GST-tagged SYT9 C2A domain with D197, 199N mutations |
| 195705 | pGEX SYT9 C2B D330, 332N | Bacterial expression plasmid encoding GST-tagged SYT9 C2B domain with D330, 332N mutations |
| 195706 | pGEX SYT9 C2AB D197,199,330,332N | Bacterial expression plasmid encoding GST-tagged SYT9 C2AB domain with D197, 199, 330, 332N mutations |
| 195707 | pGEX SYT9 C2A | Bacterial expression plasmid encoding GST-tagged SYT9 C2A domain |
| 195708 | pGEX SYT9 C2B | Bacterial expression plasmid encoding GST-tagged SYT9 C2B domain |
| 195709 | pGEX SYT9 C2AB | Bacterial expression plasmid encoding GST-tagged SYT9 C2AB domain |
| 195710 | pGEX SYT1 C2A | Bacterial expression plasmid encoding GST-tagged SYT1 C2A domain |
| 195711 | pGEX SYT1 C2B | Bacterial expression plasmid encoding GST-tagged SYT1 C2B domain |
| 195712 | pGEX SYT1 C2AB | Bacterial expression plasmid encoding GST-tagged SYT1 C2AB domain |
| 195713 | pET (SUMO)-Syt9 | Bacterial expression plasmid encoding SUMO-tagged full-length Syt9 |