|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||19639||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector typeWorm Expression ; FLP/FRT, destination vector
Growth in Bacteria
Bacterial Resistance(s)Chloramphenicol and Ampicillin, 25 & 100 μg/mL
Growth instructionsDB3.1 cells
Copy numberHigh Copy
SpeciesC. elegans (nematode), S. cerevisiae (budding yeast); Discosoma sp.
/ Fusion Protein
- 4xNLS (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ sequencing primer M13R (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Sequence mismatches between Addgene sequence and depositor sequence do not affect function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pGC320 was a gift from Jane Hubbard (Addgene plasmid # 19639 ; http://n2t.net/addgene:19639 ; RRID:Addgene_19639)
For your References section:A "FLP-Out" system for controlled gene expression in Caenorhabditis elegans. Voutev R, Hubbard EJ. Genetics. 2008 Sep . 180(1):103-19. 10.1534/genetics.108.090274 PubMed 18723890