Addgene - pLV-tetO-Klf4 Plasmid Data

Plasmid 19764: pLV-tetO-Klf4

Gene/insert name:
Klf4
Insert size:
1425
Species:
M. musculus (mouse)
Entrez Gene:
Klf4 (RP23-322L22.2, EZF, Gklf, Zie)
Vector backbone:
FUdeltaGW
(Search Vector Database)
Vector type:
Mammalian Expression, Lentiviral
Backbone size w/o insert (bp):
9941
Cloning site 5':
EcoRI
Site destroyed during cloning:
No
Cloning site 3':
EcoRI
Site destroyed during cloning:
No
5' sequencing primer:
CMV-F List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
Stbl3
Growth temperature (℃):
37
Growth instructions:
Stbl3
High or low copy:
High Copy
Sequence: View sequences (2)
Map:
View map
Principal Investigator:
Konrad Hochedlinger
Terms and Licenses:
MTA
Tet Systems Limited Use Label License

Comments:

From article: A tet-inducible lentivirus called LV-tetO was generated by removing the EcoRI/PacI fragment containing the ubiquitin promoter elements from the FUdeltaGW vector (modified from FUGW by removal of GFP with EcoRI-XbaI and re-creation of the EcoRI site) and replacing it with tetO sequences derived from pTet-Splice by EcoRI/XhoI digestion.cDNAs for c-Myc (T58A mutant), Klf4, Oct4, and Sox2 were isolated from pMIG or pMX vectors and cloned into LV-tetO using a unique EcoRI restriction site.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Defining molecular cornerstones during fibroblast to iPS cell reprogramming in mouse. Stadtfeld et al (Cell Stem Cell. 2008 Mar 6. 2(3):230-40. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 19764" in your Materials and Methods section.