Addgene - pEGFP-U6 Plasmid Data

Plasmid 19799: pEGFP-U6

Gene/insert name:
empty vector
Vector backbone:
pEGFP-N1
(Search Vector Database)
Vector type:
Mammalian Expression, RNAi
Backbone size (bp):
5000
Cloning site 5':
PmeI
Site destroyed during cloning:
No
Cloning site 3':
EcoRI
Site destroyed during cloning:
No
5' sequencing primer:
ATATCCCTTGGAGAAAAGCCTT List of Sequencing Primers
Bacterial resistance(s)
Kanamycin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (4)
Principal Investigator:
Zuoshang Xu
Terms and Licenses:
MTA

Comments:

This plasmid is modified from pEGFP-N1. Please see author's sequence for U6 modification. The modified MCS part with deletion of the fragment between Hind III and Xma I:

GCTAGCGCTACCGACTCAGATCTCGAGCTCAGATCCACCGGTCGCCACCATGGTG

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: A construct with fluorescent indicators for conditional expression of miRNA. Qiu et al (BMC Biotechnol. 2008 . 8():77. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 19799" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

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This is commonly requested with

19800: pEGFP-U6-shR-Scr

19822: pCAG-RFP-int

11619: pLB

21915: Tet-pLKO-puro

11652: pLVPT-rtTR-KRAB-2SM2