pcDNA3-FLAG-DDB1
(Plasmid #19918)

• Depositing Lab: Yue Xiong
• Publication: Hu et al Genes Dev. 2008 Apr 1. 22(7):866-71.

Backbone

• Vector backbone: pcDNA3
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5473
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: DDB1 (Damage-specific DNA binding protein 1)
• Alt name: DDB1
• Species: H. sapiens (human)
• Insert Size (bp): 3420
• GenBank ID: NM_001923
• Entrez Gene: DDB1 (a.k.a. DDBA, UV-DDB1, WHIKERS, XAP1, XPCE, XPE, XPE-BF)
• Promoter: CMV
• Tag / Fusion Protein:
  • FLAG (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: unknown (unknown if destroyed)
• 3′ cloning site: unknown (unknown if destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGH-rev

Resource Information

• Articles Citing this Plasmid:
  • 12 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Addgene QC next-generation sequencing found that the insert encodes a Leu as the terminal amino acid, rather than His found in NM_001923.