pAAV-CMV-N-HA-Pcdh15_mini_V4
(Plasmid
#199185)
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PurposeAn AAV plasmid designed to facilitate the expression of mini-PCDH15 in the inner ear of a mouse.
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 199185 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backboneAAV
- Total vector size (bp) 7580
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Vector typeAAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameHA-mmPcdh15_mini_V4-CD2
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SpeciesM. musculus (mouse)
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Insert Size (bp)3825
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Tag
/ Fusion Protein
- Human influenza hemagglutinin (HA) (N terminal on insert)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAAV-CMV-N-HA-Pcdh15_mini_V4 was a gift from David Corey (Addgene plasmid # 199185 ; http://n2t.net/addgene:199185 ; RRID:Addgene_199185) -
For your References section:
Mini-PCDH15 gene therapy rescues hearing in a mouse model of Usher syndrome type 1F. Ivanchenko MV, Hathaway DM, Klein AJ, Pan B, Strelkova O, De-la-Torre P, Wu X, Peters CW, Mulhall EM, Booth KT, Goldstein C, Brower J, Sotomayor M, Indzhykulian AA, Corey DP. Nat Commun. 2023 Apr 26;14(1):2400. doi: 10.1038/s41467-023-38038-y. 10.1038/s41467-023-38038-y PubMed 37100771
Map uploaded by the depositor.
