pFastBac Dual wt p110α/p85α
(Plasmid
#199302)
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Purposeco-expression of His-tagged p110α wt and Avi-tagged p85α wt proteins
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 199302 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepFastBac Dual
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Backbone manufacturerThermoFisher Scientific
- Backbone size w/o insert (bp) 5238
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Vector typeInsect Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameHis-tagged p110α wt and Avi-tagged p85α wt
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SpeciesH. sapiens (human)
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Entrez GenePIK3CA (a.k.a. CCM4, CLAPO, CLOVE, CWS5, HMH, MCAP, MCM, MCMTC, PI3K, PI3K-alpha, p110-alpha)
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Entrez GenePIK3R1 (a.k.a. AGM7, GRB1, IMD36, p85, p85-ALPHA, p85alpha)
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Tags
/ Fusion Proteins
- Avi (N terminal on insert)
- His
Resource Information
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A portion of this plasmid was derived from a plasmid made byGene was cloned by Peter Shepherd's Lab University of Auckland, NZ
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
received and added Avi-tag
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pFastBac Dual wt p110α/p85α was a gift from Peter Vogt (Addgene plasmid # 199302 ; http://n2t.net/addgene:199302 ; RRID:Addgene_199302) -
For your References section:
Publisher Correction: Stereo- and regiodefined DNA-encoded chemical libraries enable efficient tumour-targeting applications. Favalli N, Bassi G, Pellegrino C, Millul J, De Luca R, Cazzamalli S, Yang S, Trenner A, Mozaffari NL, Myburgh R, Moroglu M, Conway SJ, Sartori AA, Manz MG, Lerner RA, Vogt PK, Scheuermann J, Neri D. Nat Chem. 2021 Jul;13(7):714. doi: 10.1038/s41557-021-00742-x. 10.1038/s41557-021-00742-x PubMed 34103693
